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POLH Antikörper (AA 157-361)

POLH Reaktivität: Human, Ratte WB, IF, IHC, ICC, FACS Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3042492
  • Target Alle POLH Antikörper anzeigen
    POLH (Polymerase (DNA Directed), eta (POLH))
    Bindungsspezifität
    • 14
    • 5
    • 3
    • 2
    • 2
    • 1
    AA 157-361
    Reaktivität
    • 40
    • 12
    • 11
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Ratte
    Wirt
    • 38
    • 3
    Kaninchen
    Klonalität
    • 40
    • 1
    Polyklonal
    Konjugat
    • 16
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser POLH Antikörper ist unkonjugiert
    Applikation
    • 28
    • 12
    • 12
    • 10
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Verwendungszweck
    Anti-DNA polymerase eta/POLH Antibody Picoband®
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins.
    Produktmerkmale
    Anti-DNA polymerase eta/POLH Antibody Picoband® (ABIN3042492). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human POLH recombinant protein (Position: A157-R361). Human POLH shares 93.6% amino acid (aa) sequence identity with mouse POLH.
    Isotyp
    IgG
    Top Product
    Discover our top product POLH Primärantikörper
  • Applikationshinweise
    Western blot, 0.1-0.5 μg/mL, Human, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. "Entrez Gene: POLH polymerase (DNA ed), eta". 2. Johnson RE, Kondratick CM, Prakash S, Prakash L (July 1999). "hRAD30 mutations in the variant form of xeroderma pigmentosum". Science 285 (5425): 263-5. 3. Masutani C, Kusumoto R, Yamada A, Dohmae N, Yokoi M, Yuasa M, Araki M, Iwai S, Takio K, Hanaoka F (June 1999). "The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase eta". Nature 399 (6737): 700-4.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target
    POLH (Polymerase (DNA Directed), eta (POLH))
    Andere Bezeichnung
    POLH (POLH Produkte)
    Hintergrund

    Synonyms: DNA polymerase eta,2.7.7.7,RAD30 homolog A,Xeroderma pigmentosum variant type protein,POLH,RAD30, RAD30A, XPV,

    Tissue Specificity: Major component of lens fiber gap junctions.

    Background: DNA polymerase eta (Pol η), is a protein that in humans is encoded by the POLH gene. This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-ed polymerases. However, it accurately replicates UV-damaged DNA, when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.

    Molekulargewicht
    78 kDa
    Gen-ID
    5429
    UniProt
    Q9Y253
    Pathways
    DNA Reparatur
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