KIN Antikörper (AA 3-202)

Produktnummer ABIN3042474

Dieses Anti-KIN-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von KIN in WB und IHC. Geeignet für Human, Ratte und Maus.

Kurzübersicht für KIN Antikörper (AA 3-202) (ABIN3042474)

Target: KIN (KIN, Antigenic Determinant of RecA Protein Homolog (KIN))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KIN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-KIN Antikörper

Bindungsspezifität: AA 3-202

Verwendungszweck: Anti-KIN Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-KIN Antibody Picoband® (ABIN3042474). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human KIN recombinant protein (Position: K3-K202). Human KIN shares 98% amino acid (aa) sequence identity with mouse KIN.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
1. Kannouche, P., Mauffrey, P., Pinon-Lataillade, G., Mattei, M. G., Sarasin, A., Daya-Grosjean, L., Angulo, J. F. Molecular cloning and characterization of the human KIN17 cDNA encoding a component of the UVC response that is conserved among metazoans. Carcinogenesis 21: 1701-1710, 2000. 2. Timchenko, T., Bailone, A., Devoret, R. Btcd, a mouse protein that binds to curved DNA, can substitute in Escherichia coli for H-NS, a bacterial nucleoid protein. EMBO J. 15: 3986-3992, 1996.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu KIN

Target: KIN (KIN, Antigenic Determinant of RecA Protein Homolog (KIN))

Andere Bezeichnung: KIN

Hintergrund:

Synonyms: DNA/RNA-binding protein KIN17,Binding to curved DNA,KIN, antigenic determinant of recA protein homolog,KIN ,BTCD, KIN17,

Tissue Specificity: Ubiquitously expressed in all tissues examined, with highest levels in skeletal muscle, heart and testis. Differentially expressed in non-tumorigenic and tumorigenic cell lines. Highly expressed in proliferating epithelial keratinocyte cells in vitro (at protein level). .

Background: DNA/RNA-binding protein KIN17, also known as BTCD or KIN17, is a protein that in humans is encoded by the KIN gene. This gene is mapped to 10p14. The protein encoded by this gene is a nuclear protein that forms intranuclear foci during proliferation and is redistributed in the nucleoplasm during the cell cycle. Short-wave ultraviolet light provokes the relocalization of the protein, suggesting its participation in the cellular response to DNA damage. Originally selected based on protein-binding with RecA antibodies, the mouse protein presents a limited similarity with a functional domain of the bacterial RecA protein, a characteristic shared by this human ortholog. Alternative splicing of this gene results in multiple transcript variants.

Sequence Similarities: Belongs to the KIN17 family.

Molekulargewicht: 45 kDa

Gen-ID: 22944

UniProt: O60870