HSF4 Antikörper (N-Term)

Produktnummer ABIN3042461

Der Kaninchen Polyklonal Anti-HSF4-Antikörper wurde für WB validiert. Er ist geeignet, HSF4 in Proben von Human zu detektieren.

Kurzübersicht für HSF4 Antikörper (N-Term) (ABIN3042461)

Target: HSF4 (Heat Shock Transcription Factor 4 (HSF4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-HSF4 Antikörper

Bindungsspezifität: AA 149-185, N-Term

Verwendungszweck: Anti-HSF4 Antibody Picoband®

Sequenz: VQESTEARLR ELRQQNEILW REVVTLRQSH GQQHRVI

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-HSF4 Antibody Picoband® (ABIN3042461). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HSF4, different from the related mouse sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
1. Bu L, Jin Y, Shi Y, Chu R, Ban A, Eiberg H, Andres L, Jiang H, Zheng G, Qian M, Cui B, Xia Y, Liu J, Hu L, Zhao G, Hayden MR, Kong X (Jun 2002). "Mutant DNA-binding domain of HSF4 is associated with autosomal dominant lamellar and Marner cataract". Nat Genet 31 (3): 276-8. 2. Nakai A, Tanabe M, Kawazoe Y, Inazawa J, Morimoto RI, Nagata K (Jan 1997). "HSF4, a new member of the human heat shock factor family which lacks properties of a transcriptional activator". Mol Cell Biol 17 (1): 469-81. 3. Tanabe M, Sasai N, Nagata K, Liu XD, Liu PC, Thiele DJ, Nakai A (Nov 1999). "The mammalian HSF4 gene generates both an activator and a repressor of heat shock genes by alternative splicing". J Biol Chem 274(39): 27845-56.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu HSF4

Target: HSF4 (Heat Shock Transcription Factor 4 (HSF4))

Andere Bezeichnung: HSF4

Hintergrund:

Synonyms: Heat shock factor protein 4,HSF 4,hHSF4,Heat shock transcription factor 4,HSTF 4,HSF4,

Tissue Specificity: Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues. .

Background: Heat shock factor protein 4 is a protein that in humans is encoded by the HSF4 gene. It is mapped to 16q21. Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described.

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Molekulargewicht: 53 kDa

Gen-ID: 3299

Pathways: MAPK Signalweg