GAD Antikörper (AA 14-122)

Produktnummer ABIN3042415

Produktdetails anti-GAD Antikörper

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Bindungsspezifität: AA 14-122

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Anti-GAD67/GAD1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-GAD67/GAD1 Antibody Picoband® (ABIN3042415). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human GAD67 recombinant protein (Position: N14-D122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Kelly, C. D., Edwards, Y., Johnstone, A. P., Harfst, E., Nogradi, A., Nussey, S. S., Povey, S., Carter, N. D. : Nucleotide sequence and chromosomal assignment of a cDNA encoding the large isoform of human glutamate decarboxylase. Ann. Hum. Genet. 56: 255-265, 1992. 2. Bu, D.-F., Erlander, M. G., Hitz, B. C., Tillakaratne, N. J. K., Kaufman, D. L., Wagner-McPherson, C. B., Evans, G. A., Tobin, A. J. : Two human glutamate decarboxylases, 65- kDa GAD and 67- kDa GAD, are each encoded by a single gene. Proc. Nat. Acad. Sci. 89: 2115-2119, 1992. 3. "Entrez Gene: GAD1 glutamate decarboxylase 1 (brain, 67 kDa)"

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-GAD Antikörper (ABIN3042415)

Yang, Wang, He, Ding, Sun, Hua, Xi: "Dietary Restriction Affects Neuronal Response Property and GABA Synthesis in the Primary Visual Cortex." in: PLoS ONE, Vol. 11, Issue 2, pp. e0149004, (2016) (PubMed).

Details zu GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Andere Bezeichnung: GAD1 (GAD1 Produkte)

Hintergrund:

Synonyms: Glutamate decarboxylase 1,4.1.1.15,67 kDa glutamic acid decarboxylase,GAD-67,Glutamate decarboxylase 67 kDa isoform,GAD1,GAD, GAD67,

Tissue Specificity: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. .

Background: Glutamate decarboxylase 1 (brain, 67 kDa) (GAD67), also known as GAD1, is a human gene. It is mapped to 2q31.1. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Sequence Similarities: Belongs to the group II decarboxylase family.

Molekulargewicht: 67 kDa

Gen-ID: 2571

UniProt: Q99259