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GAD Antikörper (AA 14-122)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GAD in WB. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 1 Publikation erwähnt.
Produktnummer ABIN3042415

Kurzübersicht für GAD Antikörper (AA 14-122) (ABIN3042415)

Target

Alle GAD (GAD1) Antikörper anzeigen
GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reaktivität

  • 120
  • 65
  • 58
  • 6
  • 6
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 93
  • 37
  • 3
  • 2
Kaninchen

Klonalität

  • 90
  • 45
Polyklonal

Konjugat

  • 66
  • 12
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser GAD Antikörper ist unkonjugiert

Applikation

  • 93
  • 50
  • 25
  • 18
  • 17
  • 15
  • 13
  • 13
  • 13
  • 12
  • 11
  • 5
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Bindungsspezifität

    • 24
    • 15
    • 11
    • 9
    • 8
    • 8
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 14-122

    Verwendungszweck

    Anti-GAD67/GAD1 Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-GAD67/GAD1 Antibody Picoband® (ABIN3042415). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human GAD67 recombinant protein (Position: N14-D122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    1. Kelly, C. D., Edwards, Y., Johnstone, A. P., Harfst, E., Nogradi, A., Nussey, S. S., Povey, S., Carter, N. D. : Nucleotide sequence and chromosomal assignment of a cDNA encoding the large isoform of human glutamate decarboxylase. Ann. Hum. Genet. 56: 255-265, 1992. 2. Bu, D.-F., Erlander, M. G., Hitz, B. C., Tillakaratne, N. J. K., Kaufman, D. L., Wagner-McPherson, C. B., Evans, G. A., Tobin, A. J. : Two human glutamate decarboxylases, 65- kDa GAD and 67- kDa GAD, are each encoded by a single gene. Proc. Nat. Acad. Sci. 89: 2115-2119, 1992. 3. "Entrez Gene: GAD1 glutamate decarboxylase 1 (brain, 67 kDa)"

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Yang, Wang, He, Ding, Sun, Hua, Xi: "Dietary Restriction Affects Neuronal Response Property and GABA Synthesis in the Primary Visual Cortex." in: PLoS ONE, Vol. 11, Issue 2, pp. e0149004, (2016) (PubMed).

  • Target

    GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

    Andere Bezeichnung

    GAD1

    Hintergrund

    Synonyms: Glutamate decarboxylase 1,4.1.1.15,67 kDa glutamic acid decarboxylase,GAD-67,Glutamate decarboxylase 67 kDa isoform,GAD1,GAD, GAD67,

    Tissue Specificity: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. .

    Background: Glutamate decarboxylase 1 (brain, 67 kDa) (GAD67), also known as GAD1, is a human gene. It is mapped to 2q31.1. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

    Sequence Similarities: Belongs to the group II decarboxylase family.

    Molekulargewicht

    67 kDa

    Gen-ID

    2571

    UniProt

    Q99259
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