Fascin Antikörper (N-Term)

Produktnummer ABIN3042407

Der Kaninchen Polyklonal Anti-Fascin-Antikörper wurde für WB validiert. Er ist geeignet, Fascin in Proben von Human, Maus und Ratte zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für Fascin Antikörper (N-Term) (ABIN3042407)

Target: Fascin (FSCN1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Fascin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Fascin Antikörper

Bindungsspezifität: AA 42-73, N-Term

Verwendungszweck: Anti-Fascin/FSCN1 Antibody Picoband®

Sequenz: KKQIWTLEQP PDEAGSAAVC LRSHLGRYLA AD

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Fascin/FSCN1 Antibody Picoband® (ABIN3042407). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Fascin, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Saishin, Y., Shimada, S., Morimura, H., Sato, K., Ishimoto, I., Tano, Y., Tohyama, M.Isolation of a cDNA encoding a photoreceptor cell-specific actin-bundling protein: retinal fascin.FEBS Lett. 414: 381-386, 1997. 2. Wada, Y., Abe, T., Takeshita, T., Sato, H., Yanashima, K., Tamai, M.Mutation of human retinal fascin gene (FSCN2) causes autosomal dominant retinitis pigmentosa.Invest. Ophthal. Vis. Sci. 42: 2395-2400, 2001.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Fascin Antikörper (ABIN3042407)

Jiang, Wang, Chen: "Overexpression of FOXM1 is associated with metastases of nasopharyngeal carcinoma." in: Upsala journal of medical sciences, Vol. 119, Issue 4, pp. 324-32, (2014) (PubMed).

Details zu Fascin

Target: Fascin (FSCN1)

Andere Bezeichnung: FSCN1

Hintergrund:

Synonyms: Fascin,55 kDa actin-bundling protein,Singed-like protein,p55,FSCN1,FAN1, HSN, SNL,

Tissue Specificity: Ubiquitous.

Background: Fascin is an actin cross-linking protein. The Fascin gene contains 5 exons and spans 7 kb. It is a 54-58 kilodalton monomeric actin filament bundling protein originally isolated from sea urchin egg but also found in Drosophila and vertebrates, including humans. Fascin (from the Latin for bundle) is spaced at 11 nanometre intervals along the filament. The bundles in cross section are seen to be hexagonally packed, and the longitudinal spacing is compatible with a model where fascin cross-links at alternating 4 and 5 actins. It is calcium insensitive and monomeric. Fascin binds beta-catenin, and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. The role of Fascin in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective. Abnormal fascin expression or function has been implicated in breast cancer, colon cancer, esophageal squamous cell carcinoma, gallbladder cancer and prostate cancer.

Sequence Similarities: Belongs to the fascin family.

Molekulargewicht: 55 kDa

Gen-ID: 6624

UniProt: Q16658