FRZB Antikörper (AA 176-325)

Produktnummer ABIN3042406

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch FRZB in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für FRZB Antikörper (AA 176-325) (ABIN3042406)

Target: FRZB (Frizzled-Related Protein (FRZB))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FRZB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-FRZB Antikörper

Bindungsspezifität: AA 176-325

Verwendungszweck: Anti-FRZB Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-FRZB Antibody Picoband® (ABIN3042406). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human FRZB recombinant protein (Position: E176-N325). Human FRZB shares 90% amino acid (aa) sequence identity with mouse FRZB.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, -
ELISA, 0.1-0.5 μg/mL, -
1. Baker-Lepain JC, et al. Variant alleles of the Wnt antagonist FRZB are determinants of hip shape and modify the relationship between hip shape and osteoarthritis. Arthritis Rheum, 2012 May. 2. Qin S, et al. FRZB knockdown upregulates β-catenin activity and enhances cell aggressiveness in gastric cancer. Oncol Rep, 2014 May. 3. Wasmann RA, et al. Novel membrane frizzled-related protein gene mutation as cause of posterior microphthalmia resulting in high hyperopia with macular folds. Acta Ophthalmol, 2014 May.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu FRZB

Target: FRZB (Frizzled-Related Protein (FRZB))

Andere Bezeichnung: FRZB

Hintergrund:

Synonyms: Secreted frizzled-related protein 3,sFRP-3,Frezzled,Fritz,Frizzled-related protein 1,FrzB-1,FRZB,FIZ, FRE, FRP, FRZB1, SFRP3,

Tissue Specificity: Expressed primarily in the cartilaginous cores of the long bone during embryonic and fetal development and in the appendicular skeleton (6-13 weeks). At 13 weeks of gestation, transcripts were present in early chondroblasts of the tarsal bones of the foot, the carpal bones of the hands and the epiphysis of long bones. Highly expressed in placenta and heart, followed by brain, skeletal muscle, kidney and pancreas. Weakly expressed in lung and liver.

Background: FRZB is also known as FRE or OS1. The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. FRZB is a Wnt-binding protein especially important in embryonic development. It is a competitor for the cell-surface G-protein receptor Frizzled. FRZB is localized in the extracellular plasma membrane. Unlike frizzled, frzb lacks the 7 transmembrane domains normally found in G-protein-coupled receptors. It is still considered a homolog of frizzled because it contains a Cysteine Rich Domain (CRD), and because of its intracellular C-terminus which is crucial for signaling.

Sequence Similarities: Belongs to the secreted frizzled-related protein (sFRP) family.

Molekulargewicht: 36 kDa

Gen-ID: 2487

UniProt: Q92765

Pathways: WNT Signalweg, Positive Regulation of fat Cell Differentiation