Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Villin 1 Antikörper (C-Term)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Villin 1 in IHC, WB, IF und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.
Produktnummer ABIN3042343

Kurzübersicht für Villin 1 Antikörper (C-Term) (ABIN3042343)

Target

Alle Villin 1 (VIL1) Antikörper anzeigen
Villin 1 (VIL1)

Reaktivität

  • 120
  • 24
  • 19
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 60
  • 60
Kaninchen

Klonalität

  • 82
  • 38
Polyklonal

Konjugat

  • 58
  • 8
  • 6
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
Dieser Villin 1 Antikörper ist unkonjugiert

Applikation

  • 73
  • 50
  • 43
  • 19
  • 18
  • 13
  • 7
  • 5
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 58
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 770-799, C-Term

    Verwendungszweck

    Anti-Villin/VIL1 Antibody Picoband®

    Sequenz

    EQLVNKPVEE LPEGVDPSRK EEHLSIEDFT

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-Villin/VIL1 Antibody Picoband® (ABIN3042343). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminus of human Villin, different from the related mouse sequence by three amino acids.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunofluorescence, 2 μg/mL, Human, Mouse, Rat
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. Friederich, E, Vancompernolle, K, Louvard, D, Vandekerckhove, J (1999). "Villin function in the organization of the actin cytoskeleton. Correlation of in vivo effects to its biochemical activities in vitro". The Journal of Biological Chemistry274 (38): 26751-60. 2. Meng, J, Vardar, D, Wang, Y, Guo, HC, Head, JF, McKnight, CJ (2005). "High-resolution crystal structures of villin headpiece and mutants with reduced F-actin binding activity". Biochemistry 44 (36): 11963-73.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    Villin 1 (VIL1)

    Andere Bezeichnung

    VIL1

    Hintergrund

    Synonyms: Villin-1,VIL1,VIL,

    Tissue Specificity: Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes (at protein level). .

    Background: Villin is known as VIL1. This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed, they result from utilization of alternate poly-adenylation signals present in the terminal exon. In vertebrates, the villin proteins help to support the microfilaments of the microvilli of the brush border. It may play a role in cell plasticity through F-actin severing.

    Sequence Similarities: Belongs to the villin/gelsolin family.

    Molekulargewicht

    93 kDa

    Gen-ID

    7429

    UniProt

    P09327

    Pathways

    EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
Sie sind hier:
Chat with us!