PIAS1 Antikörper (AA 636-651)

Produktnummer ABIN3032369

Der Kaninchen Polyklonal Anti-PIAS1-Antikörper wurde für WB, ICC und IHC (p) validiert. Er ist geeignet, PIAS1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für PIAS1 Antikörper (AA 636-651) (ABIN3032369)

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PIAS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PIAS1 Antikörper

Bindungsspezifität: AA 636-651

Aufreinigung: Antigen affinity

Immunogen: Amino acids 636-651 (DTASIFGIIPDIISLD-human) were used as the immunogen for this PIAS1 antibody (100% homologous in human, mouse and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the PIAS1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the PIAS1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu PIAS1

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Andere Bezeichnung: PIAS1

Hintergrund: E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1(signal transducer and activator of transcription-1)] family. It contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. PIAS1 can also function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.

UniProt: O75925

Pathways: JAK-STAT Signalweg, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C