ERK1/2 Antikörper (N-Term)

Produktnummer ABIN3031825

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch ERK1/2 in WB, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für ERK1/2 Antikörper (N-Term) (ABIN3031825)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERK1/2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-ERK1/2 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: MAPK1/3 Antibody / ERK1/2

Sequenz: ARVADPDHDH TGFL

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human MAPK1/3 (ARVADPDHDHTGFL) was used as the immunogen for this MAPK1/3 antibody (100% homologous in human, mouse and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the MAPK1/3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the MAPK1/3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Andere Bezeichnung: MAPK1/3

Hintergrund: MAPK1 (ERK2) shares high homology with MAPK3 (ERK1). MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Mitogen-activated protein (MAP) kinases (also known as ERKs) have been established to function as important mediators of signal transduction by growth factor receptors. ERK1/ERK2-dependent activation of endogenous ribosomal transcription, while inactivation of ERK1/ERK2 causes an equally immediate reversion to the basal transcription level. ERK1/ERK2 was found to phosphorylate the architectural transcription factor UBF at amino acids 117 and 201 within HMG boxes 1 and 2, preventing their interaction with DNA. Mutation of these sites inhibited transcription activation and abrogated the transcriptional response to ERK1/ERK2.

UniProt: P28482