E2F2 Antikörper (C-Term)

Produktnummer ABIN3030789

This anti-E2F2 antibody (ABIN3030789) is a Rabbit Polyclonal antibody detecting E2F2 in WB. Suitable for Human, Mouse, Rat.

Kurzübersicht für E2F2 Antikörper (C-Term) (ABIN3030789)

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-E2F2 Antikörper

Bindungsspezifität: C-Term

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human E2F transcription factor 2 (ISDLFDSYDLGDLLIN) was used as the immunogen for this E2F2 antibody (100% mouse homology).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the E2F2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the E2F2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu E2F2

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Andere Bezeichnung: E2F2

Hintergrund: E2F transcription factor 2, also called E2F-2, is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The gene is mapped to 1p36 by fluorescence in situ hybridization. Electrophoretic mobility shift assays revealed the specific binding of E2F2 to ECE1b promoter sequences containing either allele of the C-338A polymorphism, with the -338A allele being associated with an increased affinity to E2F2 compared with -338C. The ability of Myc to induce S phase was impaired in the absence of either E2f2 or E2f3 but not E2f1 or E2f4. In contrast, the ability of Myc to induce apoptosis was markedly reduced in cells deleted for E2f1 but not E2f2 or E2f3.

UniProt: Q14209

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication