CAPN1 Antikörper (Middle Region)

Produktnummer ABIN3030267

Dieses Anti-CAPN1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CAPN1 in WB, ICC, IHC (p) und IHC (fro). Geeignet für Human, Maus und Ratte.

Kurzübersicht für CAPN1 Antikörper (Middle Region) (ABIN3030267)

Target: CAPN1 (Calpain 1 (CAPN1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CAPN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-CAPN1 Antikörper

Bindungsspezifität: Middle Region

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human CAPNL1 (EWNNVDPYERDQLRV) was used as the immunogen for this Calpain 1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the Calpain 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the Calpain 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu CAPN1

Target: CAPN1 (Calpain 1 (CAPN1))

Andere Bezeichnung: Calpain 1

Hintergrund: Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, CAPN1 (mu-calpain) and CAPN2 (m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L (large, catalytic, 82 kD) and S (small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits. By quantitative RT-PCR, Ueyama et al.(1998) found that expression of Calpain1/2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy (e.g., DMD, 310200) and 2 men and 3 women with amyotrophic lateral sclerosis(ALS, 105400) compared with controls. Using cDNA clones as probes, Ohno et al.(1989, 1990) assign CAPNL1 to chromosome 11.

UniProt: P07384