Beclin 1 Antikörper (AA 222-237)

Produktnummer ABIN3030154

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Beclin 1 in WB. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für Beclin 1 Antikörper (AA 222-237) (ABIN3030154)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Beclin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Beclin 1 Antikörper

Bindungsspezifität: AA 222-237

Aufreinigung: Antigen affinity

Immunogen: Amino acids 222-237 (LDQEEAQYQREYSEFK) were used as the immunogen for this Beclin 1 antibody (100% homologous in human, mouse and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Titration of the Beclin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized Beclin 1 antibody at 4°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Andere Bezeichnung: Beclin 1

Hintergrund: Bellini-1 is a protein that in humans is encoded by the BECN1 gene, also known as BECN1, ATG6, VPS30, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, autophagy-related gene 6 (ATG6). Beclin 1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy. The gene encodes a novel 450-amino acid protein containing a coiled-coil region, within which it has limited homology to myosin-like proteins. Northern blot analysis of mouse and adult human tissues revealed widespread expression. A 2.2-kb transcript was present at highest levels in human skeletal muscle and at detectable levels in all tissues examined. In some tissues, additional 1.7- and 1.4-kb transcripts were observed, suggesting the presence of alternatively spliced transcripts. Immunoperoxidase staining of human hippocampus and frontal cortex sections revealed Beclin 1 immunoreactivity in many neurons throughout these regions, as well as in some glial cells. It protects against infection by a neurovirulent strain of Sindbis virus that is known to overcome the protective effects of Bcl2. Liang et al. showed that Beclin 1 reduced Sindbis virus replication in mouse brain and reduced Sindbis virus-induced cell death in mouse brain.

UniProt: Q14457

Pathways: Autophagie