Aurora Kinase B Antikörper (N-Term)

Produktnummer ABIN3030075

Dieses Anti-Aurora Kinase B-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Aurora Kinase B in WB. Geeignet für Maus und Ratte.

Kurzübersicht für Aurora Kinase B Antikörper (N-Term) (ABIN3030075)

Target: Aurora Kinase B (AURKB)

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aurora Kinase B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Aurora Kinase B Antikörper

Bindungsspezifität: N-Term

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of mouse AURKB (QNKQPFTIDNFEIGRP) was used as the immunogen for this Aurora B antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the Aurora B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the Aurora B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Andere Bezeichnung: Aurora B

Hintergrund: Aurora kinase B, also called Aik2, AIM-1, ARK2, AurB, STK12, IPL1, PPP1R48 or STK5, localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. Cell cycle and Northern blot analyses showed that AURKB is expressed in the S phase and persistently thereafter. Western blot analysis indicated that it is localized in the midbodies during anaphase. Northern blot analysis detected strong expression of a 1.5-kb transcript in thymus, with weaker expression in small intestine, testis, colon, spleen, and brain. Examination of the role of both kinases in the phosphorylation of CENPA revealed that the reaction is mediated sequentially by AURKA and AURKB in early mitosis. EB1 overexpression enhanced AURKB kinase activity, and knockdown of EB1 with small interfering RNA impaired AURKB activity. EB1 protected Aurora B from dephosphorylation/inactivation by protein phosphatase-2A by blocking binding of PP2A.

UniProt: O70126

Pathways: T-Zell Rezeptor Signalweg, Zellzyklus, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades