beta Actin Antikörper (AA 2-19)

Produktnummer ABIN3029836

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch beta Actin in WB. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für beta Actin Antikörper (AA 2-19) (ABIN3029836)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser beta Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-beta Actin Antikörper

Bindungsspezifität: AA 2-19

Aufreinigung: Antigen affinity

Immunogen: Amino acids 2-19 (DDDIAALVVDNGSGMCKA) were used as the immunogen for this b-Actin antibody (100% homologous in human, mouse and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the b-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the b-Actin antibody can be stored for up to one month at 4 °C. For long-term, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: B-Actin

Hintergrund: B-Actin (ACTB) is one of six different isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal isoforms. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al.(1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped four ACTB pseudogenes to other chromosomes. Karakozova et al.(2006) found that arginylation of b-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al.(2006)concluded that arginylation of b-actin apparently represents a critical step in the actin N-terminal processing needed for functioning in vivo.

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction