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HLA-Aw32-&-HLA-A25 (MHC-I) Antikörper

This anti- antibody (ABIN3026979) is a Mouse Monoclonal antibody detecting in FACS, IF, IHC (p). Suitable for Human.
Produktnummer ABIN3026979

Kurzübersicht für HLA-Aw32-&-HLA-A25 (MHC-I) Antikörper (ABIN3026979)

Target

HLA-Aw32-&-HLA-A25 (MHC-I)

Reaktivität

Human

Wirt

  • 8
Maus

Klonalität

  • 8
Monoklonal

Konjugat

  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Unkonjugiert

Applikation

  • 8
  • 8
  • 7
  • 1
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon

CATA-1
  • Produktmerkmale

    This mAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31, B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This mAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.

    Aufreinigung

    Protein G affinity chromatography

    Immunogen

    Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, and DRw10 were used as the immunogen for the HLA-A25 antibody (HLA-Aw32).

    Isotyp

    IgG2a kappa
  • Applikationshinweise

    Optimal dilution of the HLA-A25 antibody (HLA-Aw32) should be determined by the researcher.

    1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1 mg/mL

    Buffer

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    Konservierungsmittel

    Azide free

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store the HLA-A25 antibody (HLA-Aw32) at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target

    HLA-Aw32-&-HLA-A25 (MHC-I)

    Andere Bezeichnung

    HLA-A25 (HLA-Aw32)

    Hintergrund

    This mAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31, B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This mAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.
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