Vimentin Antikörper

Produktnummer ABIN3026899

Der Maus Monoklonal anti-Vimentin Antikörper (Klon LN-6) (ABIN3026899) detektiert spezifisch Vimentin in IF, FACS und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Schwein, Rind (Kuh), Cat, Kaninchen und Schaf.

Kurzübersicht für Vimentin Antikörper (ABIN3026899)

Target: Vimentin (VIM)

Reaktivität: Human, Ratte, Maus, Schwein, Rind (Kuh), Cat, Kaninchen, Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: LN-6

Produktdetails anti-Vimentin Antikörper

Produktmerkmale: This mAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

Aufreinigung: PEG precipitation

Immunogen: A human thymic nuclear extract was used as the immunogen for the Vimentin antibody.

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Vimentin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Vimentin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: Vimentin

Hintergrund: This mAb reacts with a 58  kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

Pathways: Caspase Kaskade in der Apoptose