Multi Cytokeratin 4/5/6/8/10/13/18 Antikörper

Produktnummer ABIN3026733

Der Maus Monoklonal anti-Multi Cytokeratin 4/5/6/8/10/13/18 Antikörper wird verwendet zum Nachweis von Multi Cytokeratin 4/5/6/8/10/13/18 in Proben von Rind (Kuh), Frosch, Ziege, Meerschweinchen, Human, Marmoset, Maus, Schwein und Ratte. Er wurde validiert für FACS, IF, IHC (p) und WB.

Kurzübersicht für Multi Cytokeratin 4/5/6/8/10/13/18 Antikörper (ABIN3026733)

Target: Multi Cytokeratin 4/5/6/8/10/13/18

Reaktivität: Rind (Kuh), Frosch, Ziege, Meerschweinchen, Human, Marmoset, Maus, Schwein, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Klon: C11

Produktdetails

Produktmerkmale: This antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.

Aufreinigung: Protein G affinity chromatography

Immunogen: A keratin-enriched preparation from cultured human A431 was used as the immunogen for this Multi Cytokeratin antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: use the Multi Cytokeratin mAb at 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Multi Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Antigendetails

Target: Multi Cytokeratin 4/5/6/8/10/13/18

Hintergrund: This antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.