Multi Cytokeratin 4/5/6/8/10/13/18 Antikörper

Produktnummer ABIN6252020

Produktdetails

Target: Multi Cytokeratin 4/5/6/8/10/13/18

Reaktivität: Marmoset, Maus, Schwein, Rind (Kuh), Ratte, Frosch, Ziege, Meerschweinchen, Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)

Aufreinigung: Protein G affinity chromatography

Immunogen: A keratin-enriched preparation from cultured human A431 was used as the immunogen for this Multi Cytokeratin antibody.

Klon: C11

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: use the Multi Cytokeratin mAb at 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

WB: 0.5-1 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Antigendetails

Target: Multi Cytokeratin 4/5/6/8/10/13/18

Hintergrund: This antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.

Gen-ID: 3851, 3852, 3853