Involucrin Antikörper

Produktnummer ABIN3026654

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Involucrin in IF, FACS und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Hund, Schwein, Gorilla und Owl Monkey.

Kurzübersicht für Involucrin Antikörper (ABIN3026654)

Target: Involucrin (IVL)

Reaktivität: Human, Hund, Schwein, Gorilla, Owl Monkey

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Involucrin Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SY5

Produktdetails anti-Involucrin Antikörper

Keine Kreuzreaktivität: Maus

Produktmerkmale: This antibody recognizes a protein of 66-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this antibody reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between amino acids 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Aufreinigung: Protein G affinity chromatography

Immunogen: Purified Involucrin from human keratinocytes was used as the immunogen for this antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by digestion with trypsin Protease XXV (Sigma) at 1 mg/mL PBS for 5 min at 37 °C.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Involucrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Involucrin

Target: Involucrin (IVL)

Andere Bezeichnung: Involucrin

Hintergrund: This antibody recognizes a protein of 66-170  kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this antibody reacts with a 120  kDa protein. It stains the involucrin in a variety of sizes: 170  kDa in MCF-7 cells, a doublet of ~115 and 150  kDa in gorilla and owl monkey, 66  kDa in dog, and a doublet of 105  kDa in pig. Its epitope maps between amino acids 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Gen-ID: 3713