BCL2L1 Antikörper

Produktnummer ABIN3026614

Der Maus Monoklonal anti-BCL2L1 Antikörper (Klon BX006) (ABIN3026614) detektiert spezifisch BCL2L1 in WB, IF, FACS und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Schwein.

Kurzübersicht für BCL2L1 Antikörper (ABIN3026614)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BCL2L1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: BX006

Produktdetails anti-BCL2L1 Antikörper

Produktmerkmale: This antibody recognizes a protein of 27 kDa, identified as Bcl-X. The mAb shows no cross-reaction with Bcl2 alpha or Bax. Bcl-X has two isoforms, XL (long), a 241 amino acid protein which suppresses cell death, and XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl2.

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids QSNRELVVDFLS (3-14) of the human protien were used as the immunogen for this Bcl-X antibody (2).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bcl-X antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA buffer,  pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. This antibody reacts with both BclXS and BclXL.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (3)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Bcl-X antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Andere Bezeichnung: Bcl-X

Hintergrund: This antibody recognizes a protein of 27  kDa, identified as Bcl-X. The mAb shows no cross-reaction with Bcl2 alpha or Bax. Bcl-X has two isoforms, XL (long), a 241 amino acid protein which suppresses cell death, and XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl2.

Gen-ID: 598

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling