BCL2L1 Antikörper

Produktnummer ABIN3026614

Dieses Anti-BCL2L1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von BCL2L1 in WB, IF, FACS und IHC (p). Geeignet für Human, Maus, Ratte und Schwein.

Kurzübersicht für BCL2L1 Antikörper (ABIN3026614)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BCL2L1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: BX006

Produktdetails anti-BCL2L1 Antikörper

Produktmerkmale: This antibody recognizes a protein of 27 kDa, identified as Bcl-X. The mAb shows no cross-reaction with Bcl2 alpha or Bax. Bcl-X has two isoforms, XL (long), a 241 amino acid protein which suppresses cell death, and XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl2.

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids QSNRELVVDFLS (3-14) of the human protien were used as the immunogen for this Bcl-X antibody (2).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bcl-X antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA buffer,  pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. This antibody reacts with both BclXS and BclXL.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (3)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Bcl-X antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Andere Bezeichnung: Bcl-X

Hintergrund: This antibody recognizes a protein of 27  kDa, identified as Bcl-X. The mAb shows no cross-reaction with Bcl2 alpha or Bax. Bcl-X has two isoforms, XL (long), a 241 amino acid protein which suppresses cell death, and XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl2.

Gen-ID: 598

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling