GNAS Antikörper (AA 385-394)

Produktnummer ABIN302636

Der Kaninchen Polyklonal Anti-GNAS-Antikörper wurde für IHC (p) und EIA validiert. Er ist geeignet, GNAS in Proben von Human, Affe und Primate zu detektieren.

Kurzübersicht für GNAS Antikörper (AA 385-394) (ABIN302636)

Target: GNAS (GNAS Complex Locus (GNAS))

Reaktivität: Human, Affe, Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GNAS Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-GNAS Antikörper

Bindungsspezifität: AA 385-394

Spezifität: This antibody detects GNAS at amino acids 385 to 394.

Aufreinigung: Protein G column

Immunogen: Synthetic peptide corresponding to amino acids 385 to 394 of human GNAS - KLH conjugated

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on paraffin sections (20 μg/mL). ELISA (1: 0 - 1: 1000). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C for longer.

Details zu GNAS

Target: GNAS (GNAS Complex Locus (GNAS))

Andere Bezeichnung: GNAS1 / XLalphas

Hintergrund: The GNAS1 gene encodes the alpha subunit of the G protein Gs, which couples receptor binding by several hormones to activation of adenylate cyclase (Hayward et al., 1998). This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript exists, and this antisense transcript and one of the transcripts are paternally expressed, produce noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.Synonyms: Adenylate cyclase-stimulating G alpha protein, Extra large alphas protein, G protein alpha XLas, Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas

Gen-ID: 2778

NCBI Accession: NP_536350

UniProt: Q5JWF2

Pathways: Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis