NKX2-1 Antikörper (full length)

Produktnummer ABIN3025852

Der Maus Monoklonal anti-NKX2-1 Antikörper (Klon SPM150) (ABIN3025852) detektiert spezifisch NKX2-1 in IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für NKX2-1 Antikörper (full length) (ABIN3025852)

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NKX2-1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: SPM150

Produktdetails anti-NKX2-1 Antikörper

Bindungsspezifität: full length

Verwendungszweck: NKX2.1 Antibody / TTF-1 (azide and preservative free)

Aufreinigung: Protein G affinity chromatography

Immunogen: Full length rat recombinant protein was used as the immunogen for this NKX2.1 antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the NKX2.1 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the NKX2.1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu NKX2-1

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Andere Bezeichnung: NKX2.1

Hintergrund: Recognizes a protein of 40 kDa, identified as NKX2.1 or Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-NKX2.1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of NKX2.1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. Reactivity is also seen in thyroid malignancies.

UniProt: P43699

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Glucan Metabolic Process, Feeding Behaviour