Liver Arginase Antikörper (AA 1-150)

Produktnummer ABIN3025822

Dieses Anti-Liver Arginase-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Liver Arginase in WB, FACS, IHC (p) und IF. Geeignet für Human.

Kurzübersicht für Liver Arginase Antikörper (AA 1-150) (ABIN3025822)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Liver Arginase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Klon: ARG1-1125

Produktdetails anti-Liver Arginase Antikörper

Bindungsspezifität: AA 1-150

Aufreinigung: Protein G affinity chromatography

Immunogen: A recombinant fragment from amino acids 1-150 of human ARG1 was used as the immunogen for the Arginase 1 antibody.

Isotyp: IgG3 kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Arginase 1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Arginase 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Andere Bezeichnung: Arginase 1

Hintergrund: Recognizes a protein of 35-38  kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

Pathways: Cellular Response to Molecule of Bacterial Origin