BCL2L1 Antikörper (AA 3-14)

Produktnummer ABIN3025808

Dieses Maus Monoklonal-Antikörper erkennt spezifisch BCL2L1 in WB, IF, FACS und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Schwein.

Kurzübersicht für BCL2L1 Antikörper (AA 3-14) (ABIN3025808)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BCL2L1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: BX006-2H12

Produktdetails anti-BCL2L1 Antikörper

Bindungsspezifität: AA 3-14

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids 3-14 (QSNRELVVDFLS) from the human protein were used as the immunogen for this Bcl-X antibody.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the Bcl-X antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Bcl-X antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Andere Bezeichnung: Bcl-X

Hintergrund: Recognizes a protein of 27  kDa, identified as the Bcl-X protein. This mAb cocktail shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins.

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling