Nuclear Antigen Antikörper

Produktnummer ABIN3025764

Produktdetails anti-Nuclear Antigen Antikörper

Target: Nuclear Antigen (IPO-38)

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nuclear Antigen Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: PEG precipitation

Immunogen: Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the IPO-38 antibody.

Klon: IPO-38

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the IPO-38 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the IPO-38 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Nuclear Antigen

Target: Nuclear Antigen (IPO-38)

Andere Bezeichnung: IPO-38 (IPO-38 Produkte)

Hintergrund: Recognizes a protein of 14-16  kDa, which is a novel nuclear antigen of proliferating cells. IPO38 antigen is present in the nuclei of proliferating cells such as Hodgkin s disease and non-Hodgkin s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO38 may be a useful marker of cell proliferation during monitoring of tumor progression.