beta-2 Microglobulin Antikörper

Produktnummer ABIN3025748

Dieses Maus Monoklonal-Antikörper erkennt spezifisch beta-2 Microglobulin in FACS, IF, IP und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und Non-Human Primate.

Kurzübersicht für beta-2 Microglobulin Antikörper (ABIN3025748)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reaktivität: Human, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser beta-2 Microglobulin Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: BBM-1

Produktdetails anti-beta-2 Microglobulin Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: MOLT-4 human T cell line was used as the immunogen for the Beta-2 Microglobulin antibody.

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunoprecipitation: 2 μg/mg of lysate,Immunohistochemistry (Frozen): 0.5-1 μg/mL for 30 min at RT

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Beta-2 Microglobulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Andere Bezeichnung: beta-2 Microglobulin

Hintergrund: Recognizes a protein of 12  kDa, identified as microglobulin. Major histocompatibility complex (MHC) class 1 Molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 Molecules consist of an alpha- heavy chain that contains three subdomains (alpha-1, alpha-2, alpha-3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha-3 subdomain of the alpha- heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 Molecules. The alpha-1 and alpha-2 domains of the alpha- heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process