Androgen Receptor Antikörper (AA 151-350)

Produktnummer ABIN3025734

Der Maus Monoklonal Anti-Androgen Receptor-Antikörper wurde für IF, IHC (p) und FACS validiert. Er ist geeignet, Androgen Receptor in Proben von Human zu detektieren.

Kurzübersicht für Androgen Receptor Antikörper (AA 151-350) (ABIN3025734)

Target: Androgen Receptor (AR)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Androgen Receptor Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Klon: DHTR-882

Produktdetails anti-Androgen Receptor Antikörper

Bindungsspezifität: AA 151-350

Keine Kreuzreaktivität: Maus

Aufreinigung: Protein G affinity chromatography

Immunogen: A recombinant partial-length human AR protein corresponding to amino acids 151-350 was used as the immunogen for the Androgen Receptor antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Androgen Receptor antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Androgen Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Androgen Receptor

Target: Androgen Receptor (AR)

Andere Bezeichnung: Androgen Receptor

Hintergrund: Recognizes a protein of 110  kDa, which is identified as androgen receptor (AR). It reacts with full length, and the newly described A form of the receptor. It does not cross react with estrogen, progesterone, or glucocorticoid receptors. The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed, as a marker of hormone-responsiveness and thus it may be useful in identifying patients likely to benefit from anti-androgen therapy. Anti-androgen receptor has been useful clinically in differentiating morpheaform basal cell carcinoma (mBCC) from desmoplastic trichoepithelioma (DTE) in the skin. This mAb is superb for staining of formalin/paraffin tissues.

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding