Nucleolin Antikörper

Produktnummer ABIN3025707

Der Maus Monoklonal Anti-Nucleolin-Antikörper wurde für WB, IF, FACS und IHC (p) validiert. Er ist geeignet, Nucleolin in Proben von Human, Maus, Ratte, Rind (Kuh), Schwein, Kaninchen, Meerschweinchen, Huhn, Cat und Wüstenrennmaus zu detektieren.

Kurzübersicht für Nucleolin Antikörper (ABIN3025707)

Target: Nucleolin (NCL)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Kaninchen, Meerschweinchen, Huhn, Cat, Wüstenrennmaus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nucleolin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: NCL-902

Produktdetails anti-Nucleolin Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the Nucleolin antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Nucleolin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,IF: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Western blot: 0.5-1 μg/mL,Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Nucleolin

Target: Nucleolin (NCL)

Andere Bezeichnung: Nucleolin

Hintergrund: Recognizes a protein of ~76  kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

Pathways: Ribonucleoprotein Complex Subunit Organization