NUMA1 Antikörper

Produktnummer ABIN3025683

Der Maus Monoklonal anti-NUMA1 Antikörper wird verwendet zum Nachweis von NUMA1 in Proben von Human. Er wurde validiert für IHC (p).

Kurzübersicht für NUMA1 Antikörper (ABIN3025683)

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NUMA1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: A73-B-D12

Produktdetails anti-NUMA1 Antikörper

Verwendungszweck: NuMA Antibody (azide and preservative free)

Aufreinigung: PEG precipitation

Immunogen: Colon carcinoma 174T cells were used as the immunogen for the NuMA antibody.

Isotyp: IgM, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NuMA antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the NuMA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Andere Bezeichnung: NuMA

Hintergrund: Recognizes a phosphorylated protein of 228 kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200 kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

UniProt: Q14980

Pathways: Caspase Kaskade in der Apoptose, Regulation of Actin Filament Polymerization, M Phase