NUMA1 Antikörper

Produktnummer ABIN3025683

Produktdetails anti-NUMA1 Antikörper

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NUMA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: PEG precipitation

Immunogen: Colon carcinoma 174T cells were used as the immunogen for the NuMA antibody.

Klon: A73-B-D12

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NuMA antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the NuMA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Andere Bezeichnung: NuMA (NUMA1 Produkte)

Synonyme: NUMA antikoerper, 6720401E04Rik antikoerper, AA764025 antikoerper, AL022610 antikoerper, AU014979 antikoerper, NUMA1 antikoerper, NuMA antikoerper, SP-H antikoerper, numa antikoerper, numa1 antikoerper, nuclear mitotic apparatus protein 1 antikoerper, nuclear mitotic apparatus protein 1 S homeolog antikoerper, NUMA1 antikoerper, Numa1 antikoerper, numa1 antikoerper, numa1.S antikoerper

Hintergrund: Recognizes a phosphorylated protein of 228  kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200  kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

Pathways: Caspase Kaskade in der Apoptose, Regulation of Actin Filament Polymerization, M Phase