MYOD1 Antikörper

Produktnummer ABIN3025674

Der Maus Monoklonal Anti-MYOD1-Antikörper wurde für IHC (p) validiert. Er ist geeignet, MYOD1 in Proben von Human, Maus, Ratte und Huhn zu detektieren.

Kurzübersicht für MYOD1 Antikörper (ABIN3025674)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MYOD1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 5-8A

Produktdetails anti-MYOD1 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant mouse MyoD1 protein was used as the immunogen for this MyoD1 antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the MyoD1 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the MyoD1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Andere Bezeichnung: MyoD1

Hintergrund: Recognizes a phosphor-protein of 45  kDa, identified as MyoD1. The epitope of this mAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development