MYOD1 Antikörper (AA 180-189)

Produktnummer ABIN3025672

Dieses Anti-MYOD1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MYOD1 in IF, FACS und IHC (p). Geeignet für Human, Maus, Ratte und Huhn.

Kurzübersicht für MYOD1 Antikörper (AA 180-189) (ABIN3025672)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MYOD1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM427

Produktdetails anti-MYOD1 Antikörper

Bindungsspezifität: AA 180-189

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant mouse protein was used as the immunogen for this anti-MyoD1 antibody. The epitope of this mAb maps between amino acid 180-189.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the anti-MyoD1 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the anti-MyoD1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Andere Bezeichnung: MyoD1

Hintergrund: Recognizes a phosphor-protein of 45  kDa, identified as MyoD1. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas.

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development