ST6GAL1 Antikörper

Produktnummer ABIN3025660

Dieses Anti-ST6GAL1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ST6GAL1 in WB, FACS, IF und IHC (p). Geeignet für Human.

Kurzübersicht für ST6GAL1 Antikörper (ABIN3025660)

Target: ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ST6GAL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: LN1

Produktdetails anti-ST6GAL1 Antikörper

Aufreinigung: PEG precipitation

Immunogen: Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes were used as the immunogen for the CDw75 antibody.

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CDw75 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the CDw75 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu ST6GAL1

Target: ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))

Andere Bezeichnung: CDw75

Hintergrund: Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This mAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.