ST6GAL1 Antikörper

Produktnummer ABIN6940578

Der Maus Monoklonal anti-ST6GAL1 Antikörper wird verwendet zum Nachweis von ST6GAL1 in Proben von Human. Er wurde validiert für IHC, FACS und IF.

Kurzübersicht für ST6GAL1 Antikörper (ABIN6940578)

Target: ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ST6GAL1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: LN-1

Produktdetails anti-ST6GAL1 Antikörper

Verwendungszweck: CDw75 (B-Cell Marker) Antibody

Spezifität: Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This MAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by PEG precipitation.

Immunogen: Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.

Isotyp: IgM, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa or Daudi cells (IF/FACS). Germinal center B- cells in a lymph node or tonsil (IHC). Use LN1 s ability to stain surface of erythrocytes as an internal positive control.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu ST6GAL1

Target: ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))

Andere Bezeichnung: ST6GAL1

Hintergrund:

6-sialyltransferase 1 (6-ST1), Alpha 2,6 ST1, Beta galactoside alpha 2,6 sialyltransferase 1, CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, SIAT1, ST6 beta galactosamide alpha 2,6 sialyltranferase 1, ST6Gal I, ST6N

HeLa or Daudi cells (IF/FACS). Germinal center B- cells in a lymph node or tonsil (IHC). Use LN1 s ability to stain surface of erythrocytes as an internal positive control.

Molekulargewicht: 46kDa

Gen-ID: 6480, 207459

UniProt: P15907