CD5 Antikörper

Produktnummer ABIN3025590

Der Maus Monoklonal Anti-CD5-Antikörper wurde für FACS, WB, IF und IHC (p) validiert. Er ist geeignet, CD5 in Proben von Human, Ratte, Rind (Kuh), Kaninchen, Pferd, Schwein, Affe, Huhn und Meerschweinchen zu detektieren.

Kurzübersicht für CD5 Antikörper (ABIN3025590)

Target: CD5

Reaktivität: Human, Ratte, Rind (Kuh), Kaninchen, Pferd, Schwein, Affe, Huhn, Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD5 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: CD5-54-F6

Produktdetails anti-CD5 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: A peptide from the intracellular region of the human protein was used as the immunogen for this CD5 antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the CD5 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the CD5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CD5

Target: CD5

Andere Bezeichnung: CD5

Hintergrund: Recognizes a 67  kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.