WT1 Antikörper

Produktnummer ABIN3025554

Der Maus Monoklonal anti-WT1 Antikörper wird verwendet zum Nachweis von WT1 in Proben von Human und Ratte. Er wurde validiert für IHC (p).

Kurzübersicht für WT1 Antikörper (ABIN3025554)

Target: WT1 (Wilms Tumor 1 (WT1))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser WT1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: WT1-857

Produktdetails anti-WT1 Antikörper

Verwendungszweck: WT1 Antibody (azide and preservative free)

Aufreinigung: Protein G affinity chromatography

Immunogen: A recombinant human protein was used as the immunogen for the WT1 antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the WT1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
2. View the recombinant version of the < a href=../tds/recombinant-wt1-antibody-rwt1-857-v3411>WT1/857 mAb.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the WT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Andere Bezeichnung: WT1

Hintergrund: Recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

UniProt: P19544

Pathways: Tube Formation