S100A9 Antikörper

Produktnummer ABIN3025524

Der Maus Monoklonal Anti-S100A9-Antikörper wurde für IF, FACS und IHC (p) validiert. Er ist geeignet, S100A9 in Proben von Human zu detektieren.

Kurzübersicht für S100A9 Antikörper (ABIN3025524)

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A9 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: S100A9-1011

Produktdetails anti-S100A9 Antikörper

Aufreinigung: PEG precipitation

Immunogen: Recombinant human protein was used as the immunogen for the S100A9 antibody.

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the S100A9 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Formalin-fixed): 0.5-1 μg/mL for 30 min at RT (1),Not suitable for frozen tissues

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the S100A9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Andere Bezeichnung: S100A9

Hintergrund: Recognizes a 14  kDa protein, identified as S100A9 (also known as Calgranulin B or MRP14), expressed by granulocytes, monocytes and by tissue macrophages. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This mAb reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteine