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HLA-DP/DR Antikörper

This anti-HLA-DP/DR antibody (ABIN3025464) is a Mouse Monoclonal antibody detecting HLA-DP/DR in FACS, IHC (p), IF. Suitable for Human.
Produktnummer ABIN3025464

Kurzübersicht für HLA-DP/DR Antikörper (ABIN3025464)

Target

HLA-DP/DR (MHC Class II HLA-DP/DR (HLA-DP/DR))

Reaktivität

  • 15
  • 5
Human

Wirt

  • 15
Maus

Klonalität

  • 15
Monoklonal

Konjugat

  • 4
  • 4
  • 3
  • 2
  • 2
Dieser HLA-DP/DR Antikörper ist unkonjugiert

Applikation

  • 15
  • 8
  • 8
  • 4
  • 2
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Klon

Bra-14
  • Produktmerkmale

    Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DR and DP. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

    Aufreinigung

    Protein G affinity chromatography

    Immunogen

    Human REH cells were used as the immunogen for the HLA-DR antibody (HLA-DP).

    Isotyp

    IgG3 kappa
  • Applikationshinweise

    Optimal dilution of the HLA-DR antibody (HLA-DP) should be determined by the researcher.

    1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    0.2 mg/mL

    Buffer

    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store the HLA-DR antibody (HLA-DP) at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target

    HLA-DP/DR (MHC Class II HLA-DP/DR (HLA-DP/DR))

    Andere Bezeichnung

    HLA-DR (HLA-DP)

    Hintergrund

    Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DR and DP. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36  kDa) and a chain (27  kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
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