Provided assay concentrations are suggestions only, LSD1 / KDM1 antibody titration may be required for optimal results.\. Western blot: 0.5-2 μg/mL, IHC (FFPE): 5 μg/mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot the LSD1 / KDM1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
LSD1 (Lysine-specific demethylase 1), also called KDM1 (lysine (K)-specific demethylase 1A), is the first histone demethylase to be identified and regulates transcription via demethylation of lysines 4 and 9 of Histone H3. It negatively regulates pro-inflammatory cytokine expression by interacting with the genes promoter region. It also plays a role in tumor development where overexpression of LSD1 correlates with a poor prognosis. It is upregulated in cancers of the bladder, breast, lung, colon, and GI tract as well as neuroblastoma and cells of mesenchymal origin such as chondrosarcoma, osteosarcoma, rhabdomyosarcoma and Ewing's sarcoma (Bennani-Baiti et al, 2012).