Cytokeratin 7 Antikörper

Produktnummer ABIN3025130

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cytokeratin 7 in IF, FACS und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für Cytokeratin 7 Antikörper (ABIN3025130)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 7 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: KRT7-760-OV-TL12-30

Produktdetails anti-Cytokeratin 7 Antikörper

Produktmerkmale: It recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant full-length human KRT7 protein (KRT7/760) and OTN 11 cells (OV-TL12/30) were used as the immunogen for the Cytokeratin 7 antibody cocktail.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Cytokeratin 7 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Cytokeratin 7 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Andere Bezeichnung: Cytokeratin 7

Hintergrund: It recognizes an intermediate filament protein (IFP) of 55  kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).