AMPD3 Antikörper

Produktnummer ABIN3025068

Produktdetails anti-AMPD3 Antikörper

Target: AMPD3 (Adenosine Monophosphate Deaminase 3 (AMPD3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser AMPD3 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktmerkmale: It recognizes a protein of ~90 kDa, which is identified as Adenosine Monophosphate Deaminase, isoform E (AMPD3). It has 767 amino acids and is assigned an EC 3.5.4.6. It is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. This mAb shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. This mAb is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the AMPD3 antibody.

Klon: AMPD3-901

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the AMPD3 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 1-2 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunocytochemistry: 1-2 μg/mL for 30 min at RT,Immunohistology (FFPE): 2-4 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the AMPD3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu AMPD3

Target: AMPD3 (Adenosine Monophosphate Deaminase 3 (AMPD3))

Andere Bezeichnung: AMPD3 (AMPD3 Produkte)

Synonyme: ampd3 antikoerper, fi25h10 antikoerper, wu:fi25h10 antikoerper, zgc:55390 antikoerper, MGC108215 antikoerper, adenosine monophosphate deaminase 3 antikoerper, adenosine monophosphate deaminase 3b antikoerper, AMP deaminase 3 antikoerper, AMPD3 antikoerper, ampd3b antikoerper, PTRG_08116 antikoerper, ampd3 antikoerper, Ampd3 antikoerper

Hintergrund: It recognizes a protein of ~90  kDa, which is identified as Adenosine Monophosphate Deaminase, isoform E (AMPD3). It has 767 amino acids and is assigned an EC 3.5.4.6. It is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. This mAb shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. This mAb is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.