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Cytochrome C Antikörper (AA 1-80)

Dieses Anti-Cytochrome C-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cytochrome C in WB, FACS, IF und IHC (p). Geeignet für Human und Ratte.
Produktnummer ABIN3024595

Kurzübersicht für Cytochrome C Antikörper (AA 1-80) (ABIN3024595)

Target

Alle Cytochrome C (CYCS) Antikörper anzeigen
Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reaktivität

  • 207
  • 170
  • 143
  • 59
  • 53
  • 53
  • 49
  • 47
  • 41
  • 19
  • 6
  • 4
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Ratte

Wirt

  • 118
  • 107
  • 5
  • 1
Maus

Klonalität

  • 143
  • 87
  • 1
Monoklonal

Konjugat

  • 118
  • 17
  • 7
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Dieser Cytochrome C Antikörper ist unkonjugiert

Applikation

  • 181
  • 93
  • 91
  • 64
  • 42
  • 36
  • 32
  • 27
  • 22
  • 22
  • 14
  • 12
  • 12
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon

7H8-2C12-CYCS-1010
  • Bindungsspezifität

    • 42
    • 16
    • 11
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-80

    Aufreinigung

    Protein G affinity chromatography

    Immunogen

    Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon Cytochrome C (7H8.2C12) and recombinant full-length human protein (CYCS/1010) were used as the immunogen for the Cytochrome C antibody cocktail.

    Isotyp

    IgG
  • Applikationshinweise

    Optimal dilution of the Cytochrome C antibody cocktail should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    0.2 mg/mL

    Buffer

    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store the Cytochrome C antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target

    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

    Andere Bezeichnung

    Cytochrome C

    Hintergrund

    Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

    Pathways

    Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity
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