CD45RA Antikörper

Produktnummer ABIN3024414

Der Maus Monoklonal anti-CD45RA Antikörper wird verwendet zum Nachweis von CD45RA in Proben von Human. Er wurde validiert für FACS, IF, WB und IHC (p).

Kurzübersicht für CD45RA Antikörper (ABIN3024414)

Target: CD45RA

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD45RA Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: PTPRC-1131

Produktdetails anti-CD45RA Antikörper

Verwendungszweck: CD45RA Antibody (azide and preservative free)

Aufreinigung: Protein G affinity chromatography

Immunogen: Stimulated human leukocytes were used as immunogen for this CD45RA antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Due to variation in protocol and secondary antibody used, the CD45RA antibody may need to be titered for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The CD45RA antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Details zu CD45RA

Target: CD45RA

Andere Bezeichnung: CD45RA

Hintergrund: CD45RA is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50 % of peripheral CD4+ T-cells, 50 % of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

UniProt: P08575