Melanoma gp100 Antikörper

Produktnummer ABIN3024239

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Melanoma gp100 in IF, FACS und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Pferd.

Kurzübersicht für Melanoma gp100 Antikörper (ABIN3024239)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reaktivität: Human, Pferd

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Melanoma gp100 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM286

Produktdetails anti-Melanoma gp100 Antikörper

Keine Kreuzreaktivität: Ratte (Rattus)

Aufreinigung: Protein G affinity chromatography

Immunogen: Membranes from a human melanoma metastasis were used as the immunogen for this anti-gp100 antibody.

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the anti-gp100 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the anti-gp100 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Andere Bezeichnung: Gp100

Hintergrund: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.