HSPG2 Antikörper

Produktnummer ABIN3023902

Der Ratte Monoklonal anti-HSPG2 Antikörper (Klon A7L6) (ABIN3023902) detektiert spezifisch HSPG2 in IF, IHC (p) und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für HSPG2 Antikörper (ABIN3023902)

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser HSPG2 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Güteklasse: Carrier-free

Klon: A7L6

Produktdetails anti-HSPG2 Antikörper

Verwendungszweck: Perlecan Antibody (HSPG) (azide and preservative free)

Produktmerkmale: This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Aufreinigung: Protein G affinity chromatography

Immunogen: A murine EHS laminin preparation was used as the immunogen for the Perlecan antibody.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Perlecan antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Perlecan antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Andere Bezeichnung: Perlecan

Hintergrund: This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism