HSPG2 Antikörper

Produktnummer ABIN3023902

Produktdetails anti-HSPG2 Antikörper

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reaktivität: Human, Maus, Rind (Kuh), Fisch, Affe, Schwein

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser HSPG2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktmerkmale: This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Aufreinigung: Protein G affinity chromatography

Immunogen: A murine EHS laminin preparation was used as the immunogen for the Perlecan antibody.

Klon: A7L6

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Perlecan antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Perlecan antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Andere Bezeichnung: Perlecan (HSPG) (HSPG2 Produkte)

Synonyme: HSPG antikoerper, PLC antikoerper, PRCAN antikoerper, SJA antikoerper, SJS antikoerper, SJS1 antikoerper, HSPG2 antikoerper, AI852380 antikoerper, Pcn antikoerper, Plc antikoerper, per antikoerper, Per antikoerper, heparan sulfate proteoglycan 2 antikoerper, perlecan (heparan sulfate proteoglycan 2) antikoerper, HSPG2 antikoerper, hspg2 antikoerper, Hspg2 antikoerper

Hintergrund: This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism