HSPG2 Antikörper

Produktnummer ABIN3023902

Dieses Anti-HSPG2-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von HSPG2 in IF, IHC (p) und FACS. Geeignet für Human, Maus, Rind (Kuh), Fisch, Affe und Schwein.

Kurzübersicht für HSPG2 Antikörper (ABIN3023902)

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reaktivität: Human, Maus, Rind (Kuh), Fisch, Affe, Schwein

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser HSPG2 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Klon: A7L6

Produktdetails anti-HSPG2 Antikörper

Produktmerkmale: This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Aufreinigung: Protein G affinity chromatography

Immunogen: A murine EHS laminin preparation was used as the immunogen for the Perlecan antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Perlecan antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Perlecan antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Andere Bezeichnung: Perlecan (HSPG)

Hintergrund: This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism