FZD2 Antikörper (Internal Region)

Produktnummer ABIN302136

Dieses Anti-FZD2-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von FZD2 in WB, ELISA, IF und IHC. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für FZD2 Antikörper (Internal Region) (ABIN302136)

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FZD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-FZD2 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Frizzled 2

Sequenz: RYATLEHPFH CPR

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration: 2-5 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 60-65 kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24 kDa was also observed in some lysates which was successfully blocked by incubation with

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-FZD2 Antikörper (ABIN302136)

Hirano, Yonezawa, Yunoue, Habu, Uchida, Yoshioka, Kishida, Kishida, Oyoshi, Fujio, Sugata, Yamahata, Hanaya, Arita: "Immunoreactivity of Wnt5a, Fzd2, Fzd6, and Ryk in glioblastoma: evaluative methodology for DAB chromogenic immunostaining." in: Brain tumor pathology, Vol. 31, Issue 2, pp. 85-93, (2014) (PubMed).

Details zu FZD2

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Andere Bezeichnung: FZD2

Hintergrund: FZD2 , frizzled homolog 2 (Drosophila) , frizzled 2

Gen-ID: 2535, 57265, 64512

NCBI Accession: NP_001457

Pathways: WNT Signalweg