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CPE Antikörper (AA 42-453)

CPE Reaktivität: Human WB, ELISA, IP, FACS Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN299519
  • Target Alle CPE Antikörper anzeigen
    CPE (Carboxypeptidase E (CPE))
    Bindungsspezifität
    • 15
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 42-453
    Reaktivität
    • 54
    • 35
    • 33
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 52
    • 2
    Ziege
    Klonalität
    • 52
    • 2
    Polyklonal
    Konjugat
    • 24
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CPE Antikörper ist unkonjugiert
    Applikation
    • 39
    • 19
    • 13
    • 13
    • 12
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)
    Spezifität
    Recognizes human Carboxypeptidase E. Cross-reactivity: Shows less than 1 % cross-reactivity with rhCPA1, rmCPA4 and rhCPB1 in ELISA and Western Blot.
    Aufreinigung
    Affinity purified
    Immunogen
    NS0-derived recombinant human Carboxypeptidase E (aa42-453).
    Isotyp
    IgG
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  • Applikationshinweise
    Approved: ELISA (0.1 - 0.2 μg/mL), Flo, IP, WB (0.1 - 0.2 μg/mL)

    Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. ELISA: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~1 ng/well. Western Blot: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~5 ng/lane under reducing and non-reducing conditions. Flow Cytometry: (A172 cells): For intracellular staining cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μLto 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents. The detection limit is ~1 ng/well. Immunoprecipitation: Used to immunoprecipitate rhCPE from conditioned media of transfected NSO cells.
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Sterile PBS
    Konzentration
    Lot specific
    Buffer
    Lyophilized from PBS, 5 % trehalose.
    Handhabung
    Avoid freeze/thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
  • Target
    CPE (Carboxypeptidase E (CPE))
    Andere Bezeichnung
    Carboxypeptidase e / CPE (CPE Produkte)
    Hintergrund
    Name/Gene ID: CPE
    Subfamily: Metallopeptidase M14
    Family: Exopeptidase

    Synonyms: CPE, Carboxypeptidase E, CPH, Carboxypeptidase H, Enkephalin convertase
    Gen-ID
    1363
    UniProt
    P16870
    Pathways
    Peptide Hormone Metabolism, Synaptic Membrane
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