CPE Antikörper (AA 42-453)

Produktnummer ABIN299519

Der Ziege Polyklonal Anti-CPE-Antikörper wurde für WB, ELISA, IP und FACS validiert. Er ist geeignet, CPE in Proben von Human zu detektieren.

Kurzübersicht für CPE Antikörper (AA 42-453) (ABIN299519)

Target: CPE (Carboxypeptidase E (CPE))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CPE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Produktdetails anti-CPE Antikörper

Bindungsspezifität: AA 42-453

Spezifität: Recognizes human Carboxypeptidase E. Cross-reactivity: Shows less than 1 % cross-reactivity with rhCPA1, rmCPA4 and rhCPB1 in ELISA and Western Blot.

Aufreinigung: Affinity purified

Immunogen: NS0-derived recombinant human Carboxypeptidase E (aa42-453).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 0.2 μg/mL), Flo, IP, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. ELISA: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~1 ng/well. Western Blot: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~5 ng/lane under reducing and non-reducing conditions. Flow Cytometry: (A172 cells): For intracellular staining cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μLto 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents. The detection limit is ~1 ng/well. Immunoprecipitation: Used to immunoprecipitate rhCPE from conditioned media of transfected NSO cells.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handhabung: Avoid freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.

Details zu CPE

Target: CPE (Carboxypeptidase E (CPE))

Andere Bezeichnung: Carboxypeptidase e / CPE

Hintergrund: Name/Gene ID: CPE
Subfamily: Metallopeptidase M14
Family: Exopeptidase

Synonyms: CPE, Carboxypeptidase E, CPH, Carboxypeptidase H, Enkephalin convertase

Gen-ID: 1363

UniProt: P16870

Pathways: Peptide Hormone Metabolism, Synaptic Membrane