CPE Antikörper (AA 42-453)
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- Target Alle CPE Antikörper anzeigen
- CPE (Carboxypeptidase E (CPE))
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Bindungsspezifität
- AA 42-453
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Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser CPE Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)
- Spezifität
- Recognizes human Carboxypeptidase E. Cross-reactivity: Shows less than 1 % cross-reactivity with rhCPA1, rmCPA4 and rhCPB1 in ELISA and Western Blot.
- Aufreinigung
- Affinity purified
- Immunogen
- NS0-derived recombinant human Carboxypeptidase E (aa42-453).
- Isotyp
- IgG
- Top Product
- Discover our top product CPE Primärantikörper
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- Applikationshinweise
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Approved: ELISA (0.1 - 0.2 μg/mL), Flo, IP, WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. ELISA: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~1 ng/well. Western Blot: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~5 ng/lane under reducing and non-reducing conditions. Flow Cytometry: (A172 cells): For intracellular staining cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μLto 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents. The detection limit is ~1 ng/well. Immunoprecipitation: Used to immunoprecipitate rhCPE from conditioned media of transfected NSO cells. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Sterile PBS
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS, 5 % trehalose.
- Handhabung
- Avoid freeze/thaw cycles.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
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- Target
- CPE (Carboxypeptidase E (CPE))
- Andere Bezeichnung
- Carboxypeptidase e / CPE (CPE Produkte)
- Hintergrund
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Name/Gene ID: CPE
Subfamily: Metallopeptidase M14
Family: Exopeptidase
Synonyms: CPE, Carboxypeptidase E, CPH, Carboxypeptidase H, Enkephalin convertase - Gen-ID
- 1363
- UniProt
- P16870
- Pathways
- Peptide Hormone Metabolism, Synaptic Membrane
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