ABCB1 Antikörper

Produktnummer ABIN296626

Produktdetails anti-ABCB1 Antikörper

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Reaktivität: Human, Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ABCB1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Recognizes an extracellular conformational epitope of CD243, also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein), a transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds. CD243 is expressed at low levels in the cell membrane of peripheral blood leucocytes, and constitutively expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. Strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs. Can be used in a shift assay to selectively demonstrate the expression and functional activity of CD243 in a target cell. Does not cross-react with mitochondrial pyruvate carboxylase.

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Aufreinigung: Affinity purified

Immunogen: Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA.

Type of Immunogen: Cells

Klon: UIC2

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: Flo (1:50 - 1:200), IF, IHC, IHC-Fr, IHC-P, IP

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This product may benefit from antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer  pH 6.0 is recommended for this purpose (3-4). Histology Positive Control Tissue: Human kidney.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Short term: -20°C
Long term: -20°C.

Details zu ABCB1

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Andere Bezeichnung: ABCB1 / MDR1 / P Glycoprotein (ABCB1 Produkte)

Hintergrund: Name/Gene ID: ABCB1
Subfamily: ATP-binding cassette - ABCB/MDR
Family: Transporter

Synonyms: ABCB1, ABC20, Abcb1b, CLCS, Colchicin sensitivity, CD243, Doxorubicin resistance, IBD13, gp170, MDR1, Multidrug resistance protein 1, P glycoprotein, P-glycoprotein 1, P-GP, CD243 antigen, PGY1

Gen-ID: 5243

UniProt: P08183