HAND2 Antikörper

Produktnummer ABIN294970

Dieser Ziege Polyklonal Antikörper detektiert spezifisch HAND2 in WB, ELISA und IHC. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für HAND2 Antikörper (ABIN294970)

Target: HAND2 (Heart and Neural Crest Derivatives Expressed 2 (HAND2))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HAND2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-HAND2 Antikörper

Spezifität: Recognizes human HAND2. Species cross-reactivity: Mouse.

Aufreinigung: Affinity purified

Sterilität: 0.2 μm filtered

Immunogen: E. coli-derived rhHAND2.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.5-1.0 μg/mL. The detection limit for rhHAND2 and rmHAND2 is approximately 1.3 ng/well. Western Blot: 0.1-0.2 μg/mL. The detection limit for rhHAND2 and rmHAND2 is approximately 25 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 10 μg/mL to detect HAND2 in mouse embryos. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLights 557-coupled anti-goat IgG at RT in the dark. Between each step, cells were washed with PBS containing BSA.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with sterile 40-50 % glycerol, PBS.

Konzentration: Lot specific

Buffer: Lyophilized from a 0.2 um filtered solution in PBS, 5 % trehalose.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu HAND2

Target: HAND2 (Heart and Neural Crest Derivatives Expressed 2 (HAND2))

Andere Bezeichnung: DHAND / HAND2

Hintergrund: Name/Gene ID: HAND2

Synonyms: HAND2, DHand, DHAND2, Hed, Thing2, BHLHa26

Gen-ID: 9464

UniProt: P61296

Pathways: Tube Formation, Feeding Behaviour