alpha Tubulin Antikörper (AA 426-430, AA 426-450)

Produktnummer ABIN293217

Dieses Anti-alpha Tubulin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von alpha Tubulin in WB, IHC, IF, IHC (p), IP und IHC (fro). Geeignet für Human, Maus, Ratte, Schwein, Rind (Kuh), Huhn, Meerschweinchen und Frosch.

Kurzübersicht für alpha Tubulin Antikörper (AA 426-430, AA 426-450) (ABIN293217)

Target: alpha Tubulin (TUBA1)

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Huhn, Meerschweinchen, Frosch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser alpha Tubulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-alpha Tubulin Antikörper

Bindungsspezifität: AA 426-430, AA 426-450

Spezifität: Recognizes chicken tubulin alpha (MW 57kD). Causes the 10nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. Does not block microtubule assembly. Does not inhibit polymerization or depolymerization of platelet tubulin in vitro. Blocks (70-80 % ) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. Species cross-reactivity: bovine, human, porcine, rat, mouse, guinea pig, gerbil and frog.

Aufreinigung: Protein G purified

Immunogen: Native chick brain microtubules. Epitope: Localizes between aa 426-450. aa 426-430 are critical for reactivity (2). Cellular Localization: Cytoplasmic.

Type of Immunogen: Purified protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: IF, IHC, IHC-Fr, IHC-P, IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Western Blot: 1-2 μg/mL for 2hrs at RT. Immunohistochemistry (Frozen and formalin/paraffin): 1:200 for 20 min at RT. Immunoprecipitation (Native and denatured): 2 μg/mg protein lysate using Protein G. Positive control: LS174T, MAD109 cells. Skin or lung.

Kommentare:

Target Species of Antibody: Chicken

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 15 mM sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu alpha Tubulin

Target: alpha Tubulin (TUBA1)

Andere Bezeichnung: Alpha Tubulin

Pathways: Microtubule Dynamics